Gene description for Hspa8
Gene name	heat shock protein family A (Hsp70) member 8
Gene symbol	Hspa8
Other names/aliases	Hsc70
Species	Rattus norvegicus
Experiment description of studies that identified Hspa8 in Insulinoma cells
1 Experiment ID 1220 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 27872147    Organism Rattus norvegicus Experiment description Primary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. Authors Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. Journal name Diabetes. Publication year 2017 Sample Insulinoma cells Sample name INS-1E cells Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
2 Experiment ID 1221 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 27872147    Organism Rattus norvegicus Experiment description Primary Human and Rat .-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. Authors Cianciaruso C, Phelps EA, Pasquier M, Hamelin R, Demurtas D, Alibashe Ahmed M, Piemonti L, Hirosue S, Swartz MA, De Palma M, Hubbell JA, Baekkeskov S. Journal name Diabetes. Publication year 2017 Sample Insulinoma cells Sample name Rat interleukin (IL)-1. (10 units/mL) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160008: EV-METRIC:55%, 44%, 33%
3 Experiment ID 1279 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 1 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
4 Experiment ID 1280 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 2 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
5 Experiment ID 1281 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 3 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
6 Experiment ID 1282 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 1 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
7 Experiment ID 1283 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%
8 Experiment ID 1284 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 22345510    Organism Rattus norvegicus Experiment description Characterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies. Authors Palmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR. Journal name Mol Cell Proteomics. Publication year 2012 Sample Insulinoma cells Sample name NHI 6F Tu28, cytokine-stimulated Replicate 3 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV120151: EV-METRIC:14%, 0%